FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005744141

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744141
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68468791
Sequences flagged as poor quality0
Sequence length33-51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1287900.1881002981343719No Hit
CTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGGC758610.11079646491786309No Hit
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAG750900.10967040443287512No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG129200.024.7261816
TCGACGA132450.024.1195667
CGACGAG130650.023.970338
CGGTATA204600.022.1030620
ACGAGGA185100.021.00449410
GACGAGG189600.020.290629
ACGTCCG37700.020.26170232
GTTCGAC160450.019.8826945
CAGTTCG171900.019.2252923
CGAGGAT188750.019.19224411
TATAGAG265000.018.40467823
ATAGAGT266850.018.2265724
GTATAGA267850.018.05787522
ATGACGG243300.017.60849416
AACGACG85450.017.40441916
AGTTCGA192250.016.8513644
CGTTACG31850.016.64626521
CTCGGTT146900.016.5480331
TCACGCT441300.016.3669458
ACTCATT479950.016.25285535
Produced by FastQC (version 0.11.5)