FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005744144

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744144
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73307604
Sequences flagged as poor quality0
Sequence length46-51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTCTA215300.015.95954345
CATGCAT175800.011.69757659
TATATAC117300.010.93314557
ATACATG183350.010.59005456
ATATATA130400.010.3351686
TGCATTC199000.010.13032811
TACATGC201350.09.6768627
AAACTCT381950.09.56762344
TCAGACG127500.09.4947592
CCATATA126500.09.4804064
TTCCATA177350.09.1350972
AAAACTC406650.09.05850743
GCATTCC235500.08.70354212
TCCATAT181600.08.6858133
CATATAT146200.08.6799915
ATATACA155000.08.6223048
AACTAAC311100.08.57765737
TGCCCGT226100.08.56894144
GTTGTCG56300.08.5521726
CAGACGT154450.08.5518881
Produced by FastQC (version 0.11.5)