FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005744151

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744151
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82769062
Sequences flagged as poor quality0
Sequence length25-101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1428650.09.34692894-95
GATCGGA1574100.09.17417994-95
AGATCGG1798550.08.79082694-95
TAGATCG467750.08.67267994-95
TCGGAAG1449400.08.6292794-95
CGGAAGA1433200.07.969985594-95
ACACGTC517850.07.8841794-95
CGTCTGA371600.07.78646394-95
CACACGT655450.07.342623794-95
CAGATCG793150.07.259433394-95
ACGTCTG471400.07.03247494-95
CACGTCT547900.07.0165494-95
AAGATCG550800.06.56251194-95
GCACACG833500.06.430023794-95
AGAGCAC1255850.06.20105694-95
AGCACAC1080950.05.80010794-95
AAGAGCA1569150.05.745000494-95
GAGATCG795600.05.59959894-95
GAAGAGC1810700.05.55998394-95
GAGCACA1257750.05.558184694-95
Produced by FastQC (version 0.11.5)