FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005744153

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744153
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81836128
Sequences flagged as poor quality0
Sequence length25-101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA593700.09.9729494-95
ACACGTC862250.09.38288594-95
ACGTCTG735700.08.97299994-95
ATCGGAA2610050.08.8790794-95
CACGTCT860350.08.80531894-95
GATCGGA2877650.08.75574294-95
CACACGT1046700.08.58516794-95
TCGGAAG2514250.08.55231194-95
AGATCGG3233450.08.5297894-95
CGGAAGA2386000.08.51472694-95
TAGATCG931200.08.17596794-95
GCACACG1269900.07.93956694-95
CAGATCG1179350.07.755153794-95
AAGATCG962250.07.4950894-95
AGAGCAC1885250.07.42590994-95
AGCACAC1592300.07.19770594-95
GAGCACA1842700.07.07658194-95
GTCTGAA818300.06.965559594-95
AAGAGCA2268350.06.902516494-95
GAAGAGC2573800.06.87653594-95
Produced by FastQC (version 0.11.5)