FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005744157

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744157
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81307718
Sequences flagged as poor quality0
Sequence length25-101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA501500.09.9290894-95
ATCGGAA2191600.09.11592594-95
ACACGTC718300.09.11014994-95
GATCGGA2421750.09.01258694-95
ACGTCTG626300.08.85088194-95
TCGGAAG2131250.08.7431594-95
CACACGT886000.08.72719194-95
AGATCGG2723650.08.64211394-95
CGGAAGA2041950.08.44860494-95
TAGATCG783000.08.39060794-95
CACGTCT733500.08.2393594-95
GCACACG1084650.08.01660794-95
CAGATCG1076100.07.79277694-95
AAGATCG793000.07.340360694-95
AGCACAC1401900.07.04608194-95
AGAGCAC1644650.06.96782894-95
GAGCACA1625500.06.612297594-95
AAGAGCA2009550.06.548695694-95
GAAGAGC2299400.06.47019994-95
GAGATCG914000.06.267366494-95
Produced by FastQC (version 0.11.5)