FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005744159

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744159
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82966061
Sequences flagged as poor quality0
Sequence length25-51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1231450.14842816269173004No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG93000.028.572866
TCGACGA104500.025.6649367
CGACGAG109350.023.7035668
CGGTATA153300.023.20395920
GAACGAT298900.020.8371245
ACCGCGT298400.019.9591436
AGACCGC305400.019.722764
CCGCGTT308150.019.3273357
GTTCGAC136850.019.2202075
GACCGCG313350.018.9495125
CGCGTTC316600.018.8043758
CAGACCG322700.018.797763
ACGAGGA170300.018.66832510
CGTTACG42150.018.6294621
CAGTTCG152200.018.567793
GTATAGA207250.018.39040222
TATAGAG213800.017.70066623
ATGACGG191650.017.50412416
ATAGAGT218150.017.4095924
AACGACG65850.017.15218716
Produced by FastQC (version 0.11.5)