FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005744160

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744160
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82966061
Sequences flagged as poor quality0
Sequence length46-51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTCTA216050.012.91423445
ACCCGGC162150.011.933355
TCACCCG170000.011.6473923
CGGACAC157800.011.549335513
ACGGACA166450.011.27356418
CACCCGG171800.011.210934
TAGCGTA20150.010.94283937
ATAGCTC171100.010.52009139
CCGGACA176950.010.43930512
CGGACAG184000.010.24719919
GACACGG184600.010.10419815
TCAGACG154650.010.0247122
GCCCGGA203150.09.59135710
CATGCAT208250.09.5293359
ATAGCGT23800.09.45370536
CACGGAC200000.09.41617417
GGCATAG153800.09.18718135
TAGCTCT197950.09.11587540
TAATCGG93650.09.0818654
TGCCCGT231950.09.0312344
Produced by FastQC (version 0.11.5)