FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005744161

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744161
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79720486
Sequences flagged as poor quality0
Sequence length25-101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA832850.010.88257494-95
ACACGTC1216900.010.21685694-95
ACGTCTG1010700.010.15758494-95
CACGTCT1159900.09.66465394-95
CACACGT1441200.09.43134294-95
GCACACG1678800.09.29626794-95
ATCGGAA3847700.08.56831694-95
GATCGGA4230250.08.51581494-95
AGAGCAC2539600.08.41351594-95
CGGAAGA3387700.08.40339794-95
TCGGAAG3612300.08.40024394-95
AGCACAC2084600.08.36307694-95
AGATCGG4689900.08.23028794-95
TCTGAAC967150.08.14865794-95
GAGCACA2391400.08.14321794-95
GTCTGAA1061000.07.98923694-95
TAGATCG1326050.07.74850394-95
AAGATCG1488850.07.59889594-95
CAGATCG1593850.07.57849394-95
AAGAGCA3064850.07.506741594-95
Produced by FastQC (version 0.11.5)