FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005744163

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744163
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76490455
Sequences flagged as poor quality0
Sequence length25-101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1654450.09.78408394-95
ATCGGAA1488700.09.67814694-95
AGATCGG1884400.09.27357994-95
TAGATCG558950.09.0259494-95
CGTCTGA341100.08.81532494-95
TCGGAAG1471750.08.76220794-95
ACACGTC491150.08.74429494-95
CGGAAGA1416300.08.58971494-95
ACGTCTG435250.07.99822694-95
CACACGT619350.07.936851594-95
CACGTCT517150.07.80994794-95
AAGATCG583050.07.780177694-95
CAGATCG779400.07.77127894-95
GCACACG759800.07.62745394-95
AGAGCAC1220000.06.724847394-95
AGCACAC1048200.06.069117594-95
GAGATCG676950.06.06825794-95
AAGAGCA1565000.06.06344694-95
GAGCACA1225300.05.83474294-95
GAAGAGC1731900.05.812749494-95
Produced by FastQC (version 0.11.5)