FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005744165

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744165
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72823671
Sequences flagged as poor quality0
Sequence length25-51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1199710.16474176370482613No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTT968030.13292793218292992No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG845410.11609000046152575No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCGC808500.1110215935145593No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG49600.024.3145436
GAACGAT331150.020.02426545
CGGTATA85800.019.32650620
TCGACGA62800.019.096157
AGACCGC342600.018.4283314
GACCGCG341400.018.394265
ACCGCGT345250.018.2737356
CGACGAG67000.017.7647638
CAGACCG358600.017.73153
TTCGGCG258450.017.533544
AACGCTA401150.017.53295123
ACGCTAC402200.017.48717924
TCGGCGC258400.017.31111745
CCGCGTT366850.017.2282627
CGCTACC413450.016.98414225
CCGGCTT444750.016.9226232
CGCGTTC378850.016.6588138
TCACGCT297000.016.5604558
GTTCGAC73400.016.4919595
AAAACGC437600.016.16515521
Produced by FastQC (version 0.11.5)