FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005744166

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744166
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72823671
Sequences flagged as poor quality0
Sequence length46-51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA734440.10085182330344208No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAGACG224400.012.9852522
AGCTTAG292750.012.92798345
GTCACTC422350.011.7360258
GGAGGTC450500.011.6423624
GGGAGGT482450.011.4870713
GGCATAG329750.011.48360735
GGTCACT439350.011.4457947
TAGCGTA13450.011.20809637
AGGTCAC474350.011.1894336
GAGGTCA485750.011.1723785
ATAGGGG350750.011.12964138
ACTCTCC465350.010.7531111
ATATAGC61000.010.6228676
AACTCTA140600.010.56234545
GAGCTTA362350.010.50037544
ACCCGGC121450.010.2819775
CGTGGCG434400.010.1835021
TGGCATA373200.010.16470934
GTTGTCG57250.010.13969826
TGTCGTT65200.010.04213928
Produced by FastQC (version 0.11.5)