FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005744167

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744167
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76348487
Sequences flagged as poor quality0
Sequence length25-101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA949050.011.02362594-95
CGTGATA74800.010.7503783
TATCTCG78450.010.4310458
ACGTCTG1126100.010.10454594-95
ACACGTC1374000.09.93691494-95
CACGTCT1302750.09.61513294-95
CACACGT1607400.09.54682794-95
GCACACG1870800.09.09491194-95
GTCTGAA1143450.08.65027994-95
TCTGAAC1044800.08.44059294-95
AGCACAC2292900.08.38372594-95
AGAGCAC2771500.08.29445694-95
GATCGGA4688200.08.24549794-95
ATCGGAA4276000.08.21175994-95
CGGAAGA3755900.08.21136194-95
GAGCACA2615050.08.18545994-95
TCGGAAG4004050.08.10925994-95
AGATCGG5200450.08.04697494-95
TAGATCG1428350.07.93203294-95
AAGAGCA3312750.07.779054694-95
Produced by FastQC (version 0.11.5)