FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005744169

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744169
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51508711
Sequences flagged as poor quality0
Sequence length33-51
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1088240.21127300195883372No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG798980.15511551046191002No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG71200.028.3388676
TCGACGA79550.025.3925447
CGGTATA117050.024.69458420
CGACGAG81850.024.2667758
GTTCGAC86800.023.194265
CAGTTCG98700.021.0587433
AGTTCGA102600.019.9293944
GTATAGA155550.019.8405722
GAACGAT287200.019.48232345
ATAGAGT161250.019.13926724
ACGAGGA139300.018.65094810
ACCGCGT294900.018.4514776
TATAGAG173050.018.43209523
GACCGCG295250.018.361345
AGACCGC300850.018.213984
ATGACGG144000.018.15154816
CGAGGAT125450.018.03837211
TAGAGTT175700.017.6804325
CAGACCG311450.017.6374153
CCGCGTT312550.017.4023087
Produced by FastQC (version 0.11.5)