FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005744170

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744170
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51508711
Sequences flagged as poor quality0
Sequence length25-51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTCTA147650.014.23443645
CATGCAT106950.012.2445299
AGCTTAG223700.012.23191445
GGGAGGT320800.012.1500453
GGAGGTC318550.011.6636444
ATAGGGG237300.011.61486538
GGCATAG236950.011.37608335
GTCACTC317650.011.2423588
AGGTCAC340550.010.9827296
TATATAC75700.010.9396047
GGTCACT329850.010.7929367
GAGGTCA348200.010.786725
ACTCTCC342750.010.62730911
GAGCTTA258550.010.5475244
ATATATA84650.010.3680676
ATACATG128300.09.910196
TGGCATA280900.09.748198534
AACGCGC30000.09.67422215
TCACTCT377650.09.6283479
AAACTCT235650.09.58646644
Produced by FastQC (version 0.11.5)