FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005744172

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744172
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55176949
Sequences flagged as poor quality0
Sequence length25-51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG205150.012.87748745
GGCATAG220000.011.90473135
GGGAGGT345850.011.8752923
GGAGGTC323700.011.7910254
GTCACTC321000.011.5526598
ATAGGGG234750.011.50147438
GGTCACT330900.011.1802877
ACTCTCC341700.010.88373111
AGGTCAC358100.010.8342446
GAGCTTA249900.010.68764544
GAGGTCA373350.010.4520095
TGGCATA255150.010.37915334
CATAGGG262900.010.09027737
GTTGTCG33100.09.65198426
GCATAGG277700.09.6174236
TCAGACG114850.09.6012112
TCACTCT397000.09.5047889
CACTCTC394100.09.28291810
AACGCGC43350.09.03037515
GCATTCG57750.09.0017973
Produced by FastQC (version 0.11.5)