FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005744175

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744175
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76652884
Sequences flagged as poor quality0
Sequence length25-101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA438350.09.81781494-95
ACACGTC630850.09.24472394-95
ATCGGAA1940700.08.990190594-95
GATCGGA2129050.08.92757794-95
ACGTCTG542000.08.78308894-95
AGATCGG2399750.08.73339894-95
TCGGAAG1878700.08.64308594-95
CACACGT772550.08.51222694-95
CGGAAGA1792300.08.48268194-95
TAGATCG730500.08.47522294-95
CACGTCT643000.08.18090594-95
GCACACG914950.07.884485294-95
CAGATCG940950.07.58113494-95
AAGATCG691700.07.467855594-95
AGAGCAC1463450.07.155560594-95
GAGCACA1438050.06.636661594-95
AGCACAC1255650.06.566550794-95
GAAGAGC2022150.06.47567394-95
AAGAGCA1838650.06.370384794-95
GAGATCG791450.06.323371494-95
Produced by FastQC (version 0.11.5)