FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005744177

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744177
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61487335
Sequences flagged as poor quality0
Sequence length50-51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTG736620.11980028082205871No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG727900.1183821025907205No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG66450.027.494056
TCGACGA77250.023.8832287
CGACGAG77100.023.6378488
CGGTATA118800.022.40503320
GTTCGAC89100.020.4546995
ACGAGGA125450.019.06518210
GTATAGA152400.018.60213922
AACGACG50200.018.60037216
CAGTTCG106650.018.4811523
AGACCGC121350.018.2819654
ACCGCGT122900.018.0876736
ATGACGG140000.017.99978316
AACGCTA223950.017.94352123
ACGCTAC226100.017.78284624
ATAGAGT158900.017.74207724
CGTTACG32700.017.40804721
TCACGCT238200.017.3329148
CAGACCG130700.017.1634793
CGCTACC234250.017.11612325
GACGAGG142650.016.9083549
Produced by FastQC (version 0.11.5)