FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005744178

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744178
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61487335
Sequences flagged as poor quality0
Sequence length25-51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTG636200.10346846224511112No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT38300.016.80086911
TCGTGTA42700.015.49111114
AACTCTA153800.014.41130745
TAGCGTA15200.013.17615337
GTCGTGT49750.012.97932913
ACCCGGC106250.012.7476655
GGTCGCC52750.012.41376543
GGCATAG172200.011.61706335
ATAGCGT18550.011.40280336
AGCTTAG184000.011.28774945
ATAGCTC114450.011.14792239
CACCCGG122600.011.1393884
TCACCCG127900.010.9944083
CGTGGCG291500.010.9136331
TCAGACG138750.010.8643522
TGTAGGG61500.010.68231917
TGCCCGT285600.010.66774644
GTCACTC257600.010.5508138
GTGGCGA296950.010.5391562
CATGCAT151750.010.5119119
Produced by FastQC (version 0.11.5)