FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005744180

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744180
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55586823
Sequences flagged as poor quality0
Sequence length25-51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA835830.15036477260087341No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG565960.10181549681297669No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG222800.013.94779745
ATAGGGG235050.013.60224438
GGCATAG234300.013.18541235
GGGAGGT340250.013.0230933
GGAGGTC327800.012.9135054
GAGCTTA251450.012.33982244
TGGCATA263450.011.86294534
CATAGGG272750.011.40042937
GTCACTC370150.011.2769738
GCATAGG284950.010.92040736
GGTCACT374450.010.9136657
GTTGTCG31350.010.76497126
ACTCTCC388000.010.66933611
TCAGACG95000.010.138542
CACTCTC423400.09.70325110
AGGGGAG457550.09.16122140
GAGGTCA477400.09.15908055
GCTCTGT464150.09.0157222
TAGGGGA458250.09.01465939
TCACTCT469550.09.0089199
Produced by FastQC (version 0.11.5)