FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005744181

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744181
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80163718
Sequences flagged as poor quality0
Sequence length25-101
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA600500.010.24598494-95
ACACGTC868450.09.52562894-95
ACGTCTG739400.09.18760994-95
GATCGGA3058900.09.07944494-95
CACACGT1049300.08.96611994-95
ATCGGAA2763750.08.92700394-95
CACGTCT859350.08.73873294-95
TAGATCG986450.08.66068594-95
AGATCGG3424600.08.6591694-95
TCGGAAG2638450.08.63580294-95
CGGAAGA2479350.08.49704594-95
GCACACG1264450.08.25571494-95
AGAGCAC1899550.07.81323894-95
CAGATCG1278800.07.655300694-95
AAGATCG1042050.07.578239494-95
AGCACAC1600550.07.398984494-95
GAGCACA1838450.07.078246694-95
GTCTGAA824400.07.01115794-95
AAGAGCA2329900.07.00495894-95
GAAGAGC2635200.06.856387694-95
Produced by FastQC (version 0.11.5)