FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005744183

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744183
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73907113
Sequences flagged as poor quality0
Sequence length25-101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA403200.09.53030494-95
ATCGGAA1816200.09.43349694-95
GATCGGA2008750.09.22864494-95
ACACGTC578350.09.16806894-95
AGATCGG2277800.08.90607594-95
TAGATCG664950.08.85042894-95
TCGGAAG1768900.08.76593694-95
ACGTCTG495600.08.58585994-95
CACACGT719300.08.55468294-95
CGGAAGA1694100.08.31175794-95
GCACACG863250.08.06721694-95
CACGTCT585300.07.89599894-95
AAGATCG703400.07.6817294-95
CAGATCG870250.07.541546394-95
AGCACAC1157100.07.09307394-95
AGAGCAC1382700.07.083287794-95
GAGCACA1360100.06.573153594-95
GAGATCG766500.06.541248394-95
GAAGAGC1942350.06.445925794-95
AAGAGCA1733500.06.415397694-95
Produced by FastQC (version 0.11.5)