FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005744185

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744185
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81688670
Sequences flagged as poor quality0
Sequence length25-101
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA627050.09.92409494-95
ACACGTC903850.09.06703194-95
ATCGGAA2788900.08.90017394-95
GATCGGA3085250.08.89324194-95
ACGTCTG770400.08.758183594-95
CACACGT1100700.08.66036494-95
AGATCGG3447050.08.43141894-95
CACGTCT896900.08.416182594-95
TCGGAAG2682100.08.37039394-95
TAGATCG935050.08.15381194-95
CGGAAGA2529950.08.07691894-95
GCACACG1338550.07.96378694-95
CAGATCG1323600.07.80501594-95
AGAGCAC1949050.07.67255694-95
AAGATCG1000000.07.53682194-95
AGCACAC1638250.07.330263694-95
GTCTGAA839650.07.161486694-95
AAGAGCA2339400.07.123334494-95
TCTGAAC783200.07.06017294-95
GAAGAGC2684950.06.925966794-95
Produced by FastQC (version 0.11.5)