FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005744187

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744187
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75136564
Sequences flagged as poor quality0
Sequence length25-101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA482650.09.59892294-95
ACACGTC710150.09.57784294-95
ATCGGAA2180250.09.06556194-95
GATCGGA2401350.08.73971894-95
ACGTCTG605700.08.738831594-95
CACACGT877200.08.7111594-95
TCGGAAG2125150.08.60980994-95
AGATCGG2708100.08.56130394-95
CACGTCT716400.08.55657694-95
TAGATCG748350.08.38859694-95
CGGAAGA2024950.08.326591594-95
GCACACG1073950.08.11283994-95
CAGATCG1056550.07.78054694-95
AAGATCG787300.07.46972294-95
AGAGCAC1612150.07.453793594-95
AGCACAC1362800.07.170944794-95
AAGAGCA1954650.06.889886494-95
GAGCACA1575400.06.77668194-95
GTCTGAA687900.06.55387394-95
GAAGAGC2243950.06.508735794-95
Produced by FastQC (version 0.11.5)