FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005744189

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744189
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79011706
Sequences flagged as poor quality0
Sequence length25-101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1677650.09.45843894-95
ATCGGAA1516750.09.35305894-95
AGATCGG1904800.09.001368594-95
ACACGTC510950.08.87469194-95
TAGATCG603400.08.75788694-95
TCGGAAG1497900.08.6715894-95
CGTCTGA358950.08.66291294-95
CGGAAGA1444400.08.24587194-95
CAGATCG765350.07.92607794-95
ACGTCTG456100.07.72208794-95
CACGTCT552800.07.49838794-95
CACACGT651100.07.434013494-95
GCACACG783000.07.191130694-95
AAGATCG611400.07.176007794-95
AGAGCAC1269200.06.527298594-95
AGCACAC1089150.06.02786794-95
AAGAGCA1605600.05.853192394-95
GAAGAGC1775950.05.85211294-95
GAGATCG642800.05.815783594-95
GAGCACA1277500.05.762339694-95
Produced by FastQC (version 0.11.5)