FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005744191

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744191
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63440291
Sequences flagged as poor quality0
Sequence length49-51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG37150.022.4679496
ACCGCGT96850.018.2355296
TCGACGA46700.017.8733237
CGACGAG46300.017.396048
GACCGCG101200.017.3182985
AGACCGC104250.017.0705994
CCGCGTT106950.016.492397
CGGTATA72300.016.30576920
GTTCGAC51100.016.2022465
CGCGTTC111400.015.8133868
ACGAACG51450.015.3936131
CAGACCG118200.015.2652953
AACGACG30450.014.55550416
GTCCGTT48150.014.3446651
CGAACGA55200.014.22556732
AACGCTA89450.013.90891123
GAACGAT131700.013.73763245
ACGCTAC93850.013.35270424
CCCGTAT40100.013.2969581
GTTCCTA112200.013.15404315
Produced by FastQC (version 0.11.5)