FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005744193

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744193
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82871860
Sequences flagged as poor quality0
Sequence length25-101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1326200.010.07420394-95
ATCGGAA1194800.09.78195394-95
AGATCGG1523100.09.58097994-95
TAGATCG482800.09.48865494-95
TCGGAAG1205750.08.80714994-95
ACACGTC423550.08.28706594-95
CGGAAGA1184350.08.07403394-95
CAGATCG626550.07.509275494-95
AAGATCG530500.07.338258394-95
CGTCTGA302550.07.299614494-95
CACACGT545700.07.154963594-95
ACGTCTG389200.06.90256694-95
CACGTCT461850.06.839232494-95
GCACACG651800.06.72360294-95
GAGATCG590000.06.13458294-95
AGAGCAC1112500.05.55950394-95
GAAGAGC1592500.05.324852594-95
AGCACAC978700.05.28688994-95
AAGAGCA1459850.05.072994794-95
GAGCACA1140450.05.019284794-95
Produced by FastQC (version 0.11.5)