FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005744195

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744195
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77844209
Sequences flagged as poor quality0
Sequence length25-101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA465200.09.91521594-95
ATCGGAA2086100.09.45446794-95
GATCGGA2301950.09.12208894-95
ACACGTC679900.09.02708494-95
TCGGAAG2025700.08.94778594-95
AGATCGG2603250.08.82247994-95
TAGATCG736000.08.80382594-95
ACGTCTG586900.08.77304894-95
CACACGT836150.08.6196394-95
CGGAAGA1920200.08.51236594-95
CACGTCT682550.08.2614694-95
GCACACG1014450.07.836253694-95
CAGATCG982050.07.643110394-95
AAGATCG792800.07.44901994-95
AGAGCAC1558950.07.21743994-95
AGCACAC1318550.06.86008894-95
GAGCACA1521950.06.67475694-95
AAGAGCA1913300.06.635345594-95
GAGATCG901000.06.53838794-95
GAAGAGC2178700.06.50969694-95
Produced by FastQC (version 0.11.5)