FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005744197

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744197
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73953703
Sequences flagged as poor quality0
Sequence length31-51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1482940.20052275137595207No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG72000.024.1266
GAACGAT371550.021.46169745
TCGACGA83100.020.7940337
CGGTATA105300.019.87114120
CGACGAG81200.019.64578
GTTCGAC86300.018.434435
GACCGCG416450.017.9929525
ACCGCGT419350.017.9374336
AGACCGC425800.017.9149554
CAGACCG443250.017.356933
CCGCGTT438300.017.1764347
CGCGTTC445500.016.918998
ACGCGTC26900.016.47791140
AACGCTA314450.016.45684223
ACGCTAC315000.016.43567324
AGTTCGA100100.016.3426344
CGCTACC323550.015.99439725
AGAACGA501900.015.95458444
CAGTTCG110100.015.8188973
CCGGCTT372400.015.6862792
Produced by FastQC (version 0.11.5)