FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005744201

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744201
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58471796
Sequences flagged as poor quality0
Sequence length32-51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT2092430.3578528697835791No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1191090.20370333758860426No Hit
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAG911160.15582897436569246No Hit
CTCAGTTCGACGAGGATGACGGTATAGAGTTTTCACTCATTTTGTTCAGCT829050.1417863066836531No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA771420.13193027284470618No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG764070.1306732565560326No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG159200.032.377786
TCGACGA166250.031.0603927
GTTCGAC170050.030.4172275
CGACGAG167100.030.363798
CGGTATA241300.028.41918220
AGTTCGA190300.027.3225144
CAGTTCG198400.026.5132123
GACGAGG235550.026.191989
ACGAGGA245950.025.37719710
GTATAGA304250.024.00363722
CGAGGAT239450.023.95184511
ATGACGG270550.023.4756316
TATAGAG319350.023.0590223
ATAGAGT320500.022.8008624
ACTCATT497500.021.1349135
GGTATAG339950.020.27151921
GATGACG306500.019.9587215
GACCGCG578750.019.950465
AGACCGC585300.019.9425624
ACCGCGT583150.019.9237486
Produced by FastQC (version 0.11.5)