FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005744205

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744205
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57218275
Sequences flagged as poor quality0
Sequence length32-51
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1112160.1943714661093855No Hit
GCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCAGGG692190.12097358754698566No Hit
CTCCAGCTTAACGGTATTTGGAGGTCAGCACGGTGCTCACAGAAGCCAGGA609830.1065795849315625No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCCTA149050.017.66064515
CCTACTC149400.017.60428818
ACCGCGT315950.017.4253526
GACCGCG316200.017.3969945
AGTTACC148900.017.37769934
AGACCGC321800.017.1642554
CAGACCG330900.016.8146133
CCGCGTT339850.016.1941997
CGCGTTC346300.015.899078
TCAGACC379300.015.024912
CTACTCT178100.014.96953219
GAACGAT372650.014.7805245
GTTACCA181200.014.59049135
TTCCTAC181500.014.58990716
GTCCGAT24150.014.3470041
ACATGCC201700.013.9992186
GCGTTCT399350.013.8658439
AACATGC204350.013.729325
TCCTACT195550.013.59918117
CCTGAAC221350.013.51853751
Produced by FastQC (version 0.11.5)