FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005744209

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744209
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77562658
Sequences flagged as poor quality0
Sequence length25-101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG67050.013.53949212-13
AATCTCG141650.012.7247868
CTCGTAT79750.011.71070510-11
CGTATGC85150.011.16121112-13
CGTCTGA1375000.011.11109994-95
ACGTCTG1626350.010.35536394-95
TCTGAAC1305850.010.16940994-95
GTCTGAA1472400.09.87656494-95
CACGTCT1866650.09.80878694-95
TATGCCG96650.09.63750214-15
ACACGTC1995650.09.56070894-95
CTGAACT1295250.09.48580994-95
ATCTCGT206450.09.3507929
CACACGT2294950.09.27908994-95
TCCAGTC757400.09.16808994-95
GCACACG2672550.08.75685194-95
TGAACTC1324550.08.40806294-95
AGCACAC3089500.08.30237794-95
AGAGCAC3717750.08.27178694-95
AACTCCA1074100.08.25766594-95
Produced by FastQC (version 0.11.5)