FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005744211

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744211
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74002279
Sequences flagged as poor quality0
Sequence length25-101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA859500.010.71936694-95
ACGTCTG1023900.010.22501494-95
ACACGTC1240300.09.85791794-95
CACGTCT1190950.09.73905494-95
CACACGT1456450.09.27119994-95
GCACACG1701850.09.0685394-95
TCGTATG67450.09.0113412-13
CTCGTAT70500.08.25246710-11
TCTGAAC951700.08.22178794-95
ATCGGAA3670150.08.21941594-95
GTCTGAA1055050.08.21920894-95
TCGGAAG3461200.08.16625994-95
AGCACAC2058750.08.02819294-95
CGGAAGA3251000.08.00836594-95
GATCGGA4013050.07.986601494-95
CGTATGC75950.07.878271612-13
AGATCGG4437700.07.84233794-95
AGAGCAC2459600.07.84192494-95
GAGCACA2327550.07.54292894-95
TAGATCG1197800.07.4801994-95
Produced by FastQC (version 0.11.5)