FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005744213

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744213
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59840742
Sequences flagged as poor quality0
Sequence length49-51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG32000.019.7462946
AACGCTA123150.017.87629723
ACGCTAC126500.017.40289124
CGGTATA53000.017.39551220
CGCTACC130150.016.93211225
TCGACGA39550.015.692497
ACCGCGT57700.015.4719116
AAAACGC145200.015.44037321
GACCGCG58050.015.3398895
GTCCGTT47500.015.0782861
GTTCGAC40900.015.00958255
CGACGAG39750.014.8781138
GTTCCTA109900.014.62977215
CTCCGGT81900.014.5566141
CCGTACG59550.014.2737894
TTCACGC170050.014.25513657
GAAAACG158150.014.23292420
CTCGGTT114200.014.1905231
TCACGCT169350.014.1281638
CGTACGC59900.014.1153055
Produced by FastQC (version 0.11.5)