FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005744214

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744214
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59840742
Sequences flagged as poor quality0
Sequence length50-51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGG131350.012.17971938
GGCATAG134050.011.51488835
AGCTTAG144100.010.97881545
CATAGGG145850.010.64501337
CACCGAA169250.010.07870445
GCATAGG155500.09.94077236
GCACCGA173100.09.72452344
GAGCTTA166900.09.60035844
CGTGTAG180200.09.36483836
GGGAGGT242800.09.3403193
GTCACTC222950.09.15321358
TCTATAG190450.09.0253113
GGAGGTC250550.08.9526524
GTTCTAT140050.08.9157881
TGGCATA182100.08.71127234
ACTCTCC257950.08.46075111
TTACTAG203750.08.39200714
GGTCACT243450.08.3454897
TACTAGA206250.08.34482615
CTATAGT208050.08.3159064
Produced by FastQC (version 0.11.5)