FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005744218

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744218
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54208528
Sequences flagged as poor quality0
Sequence length25-51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA579000.10680976247870078No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA573360.1057693357768357No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTCTA241550.020.7120645
CATGCAT156100.017.179489
CGGTATA26700.016.68364520
TATATAC119400.016.4494197
AAACTCT339300.015.14295144
TTCCATA165600.014.8248112
CCATATA136200.014.5384834
ATATATA142350.014.4137966
TCCATAT169900.014.1844623
AAAACTC363800.014.17264443
ATACATG194100.014.0017536
TGCATTC202300.013.42295211
TACATGC201700.013.3960797
CATATAT150800.013.2941285
ATATACA156800.012.7840438
GAGTGAA430600.012.45273538
ACATGCA227600.012.2965928
TCGACGA34950.012.2305797
AATGAGT458450.011.81368135
GCATTCC238150.011.79908912
Produced by FastQC (version 0.11.5)