FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005744219

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744219
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32671318
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG28950.026.58026545
CGTATGC33700.022.9375846
CTCGTAT34400.021.86166244
TAACTCG35300.021.6006935
TATGCCG36750.021.03404448
ACTCGAT45700.016.68474237
TCTCGTA47750.015.89601143
AACTCGA53450.014.59272436
ACCTAAC60200.013.0735432
TCCGATC30250.012.9503996
ATCTCGT64400.011.89482542
ATGCCGT68850.011.78614849
GATCGGA175000.011.17326870
GTATGCC70250.011.15287647
AGATCGG207400.010.99626870
CGTCTGA74750.010.76205416
ACACGTC77950.010.05102713
TTCCGAT38950.09.9679555
ACGTCTG82250.09.95082715
TAGATCG73350.09.91904270
Produced by FastQC (version 0.11.5)