FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005744221

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744221
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75762484
Sequences flagged as poor quality0
Sequence length25-101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA436250.09.57670894-95
ACACGTC634000.09.3498194-95
GATCGGA2144800.09.27747794-95
ATCGGAA1939500.09.21235394-95
AGATCGG2430800.08.91416694-95
TCGGAAG1882200.08.84938194-95
TAGATCG718900.08.72923994-95
ACGTCTG551950.08.69779694-95
CGGAAGA1798750.08.67363894-95
CACACGT776900.08.44674794-95
CACGTCT644450.08.22712894-95
GCACACG939550.08.05143694-95
CAGATCG911200.07.83453894-95
AAGATCG781650.07.498423694-95
AGAGCAC1474600.07.092827394-95
AGCACAC1253000.06.985316394-95
GAGCACA1458950.06.638689594-95
AAGAGCA1802800.06.44280494-95
GAAGAGC2055550.06.430039494-95
GAGATCG801750.06.237179894-95
Produced by FastQC (version 0.11.5)