FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005744225

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744225
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77642942
Sequences flagged as poor quality0
Sequence length25-101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1846600.09.35623394-95
GATCGGA2037500.09.30219394-95
CGTCTGA420250.09.25513394-95
ACACGTC603000.09.14816394-95
AGATCGG2314350.09.12586994-95
TAGATCG696150.08.90783594-95
TCGGAAG1803500.08.81395394-95
ACGTCTG527150.08.51299894-95
CACACGT741900.08.26504694-95
CGGAAGA1721650.08.17893694-95
CACGTCT626250.07.99181594-95
GCACACG895700.07.936295594-95
AAGATCG741550.07.571424594-95
CAGATCG861400.07.463979294-95
AGAGCAC1420800.07.08989494-95
AGCACAC1213900.06.865270694-95
GAGATCG796250.06.64846494-95
GAGCACA1429350.06.580481594-95
GAAGAGC1987050.06.38557494-95
AAGAGCA1776750.06.29404694-95
Produced by FastQC (version 0.11.5)