FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005744228

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744228
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63598967
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTCTA163400.013.94582745
ATAGGGG143750.010.23575138
CATGCAT128150.010.1462199
GGCATAG142900.09.76268435
TATATAC98200.09.5434827
GTTGTCG38950.09.51571826
CGTAACG18900.09.51232342
ATACATG148800.08.7381596
TACATGC150850.08.7096657
GTAACGA91700.08.69609318
TACGGTT40250.08.65118635
ATATATA117800.08.6490696
AGCTTAG172450.08.54867945
TAATCGG97500.08.5181394
TTCCATA141900.08.4849152
TGCATTC152100.08.418035511
CCATATA109600.08.3230744
AAACTCT296700.08.30897544
GGGAGGT263700.08.2713083
CATAGGG169300.08.26686737
Produced by FastQC (version 0.11.5)