FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005744229

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744229
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79749006
Sequences flagged as poor quality0
Sequence length25-101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA2260400.09.34098494-95
ATCGGAA2042800.09.30888294-95
CGTCTGA451800.09.211534594-95
ACACGTC660300.09.1007294-95
AGATCGG2556300.08.96439594-95
TAGATCG714850.08.77313994-95
TCGGAAG1984550.08.6991394-95
ACGTCTG571550.08.63801994-95
CACGTCT676850.08.51625394-95
CGGAAGA1902900.08.51048194-95
CACACGT818550.08.15463394-95
CAGATCG1017350.08.15326294-95
GCACACG986850.07.99929994-95
AAGATCG761700.07.700013694-95
AGAGCAC1529750.07.59268994-95
AGCACAC1294550.07.108715594-95
GAGCACA1514750.06.868743494-95
AAGAGCA1900600.06.725334694-95
GAGATCG923300.06.545859394-95
GAAGAGC2153550.06.38372994-95
Produced by FastQC (version 0.11.5)