FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005744239

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744239
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72373455
Sequences flagged as poor quality0
Sequence length31-51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1781350.2461330608024724No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG964970.13333203451458825No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTT806330.11141239560830693No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG740900.1023717881093282No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG60250.027.1507116
TCGACGA72000.022.6559247
GAACGAT440200.021.87152345
CGACGAG76650.021.0466338
CGGTATA103000.020.57694620
GTTCGAC85150.019.264895
AGACCGC493100.018.6780014
GACCGCG492100.018.2906975
ACCGCGT497500.018.1637086
CAGACCG518400.017.8836653
AACGCTA450200.017.881523
ACGCTAC451100.017.84141524
CCGGCTT484100.017.6588232
CGCTACC459500.017.49567225
TCACGCT336850.017.3731868
CCGCGTT520950.017.3622597
CGCGTTC528050.017.1245298
GCCGGCT445550.016.8832051
TCAGACC587600.016.8805792
AGAACGA577200.016.71871844
Produced by FastQC (version 0.11.5)