FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005744242

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744242
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56543824
Sequences flagged as poor quality0
Sequence length25-51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA604100.1068374859118124No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTCTA282600.021.62150245
CGGTATA38750.021.59793720
CATGCAT180200.018.465079
TATATAC138500.017.1535977
GACGGTA51500.015.98866618
AAACTCT391400.015.91016144
ATACATG231700.014.8173816
AAAACTC427650.014.75092743
ATATATA171050.014.7048876
TCGACGA42400.014.6978887
TACATGC230450.014.643867
TTCGACG41200.014.470776
TTCCATA206350.014.4707172
TGCATTC233450.014.40737611
ACATGCA250650.013.8944348
TCCATAT211800.013.8643213
CCATATA174500.013.4750214
CAATAGG114600.013.43252844
ATATACA185550.013.4100928
CATATAT184650.013.3915045
Produced by FastQC (version 0.11.5)