FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005744243

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744243
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48484261
Sequences flagged as poor quality0
Sequence length49-51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3959140.8165825194283151No Hit
CCCAGCTTCATCTTCAACGTTGTGGAAAGGGACTGTACATCATGGGGCAGA1197280.2469419921652513No Hit
CTCAGTTCGACGAGGATGACGGTATAGAGTTTTCACTCATTTTGTTCAGCT1139120.2349463468155161No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT817980.16871041924306115No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG645140.13306173729243806No Hit
CCCAGCTTTATTTCCAACGTTGTGGAAAGGGACTTGTACATCATGGGGCAG635830.13114152652548422No Hit
CTCAGTTCCGATGAGAACGACGGTATGAGTTCTCACTCATTTTGTTCAGCT600690.12389381370585394No Hit
CTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACG573710.1183291212791714No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG213700.033.651126
TCGACGA227750.031.752897
CGCGCTA3900.031.71200423
GTTCGAC232650.031.3740865
CGACGAG231950.030.974348
ACGAGGA311400.029.06517210
GACGAGG307450.029.029019
AGTTCGA253700.028.6645534
CGAGGAT297300.028.21236811
CGGTATA344900.027.94374320
GTTCCGA182450.027.1516515
CAGTTCG270700.027.1286133
AGTTCCG182800.027.0873644
CGGTATG221750.026.77108221
GTATAGA384450.026.53131722
TATAGAG404750.026.25067923
AACGACG165400.026.15743616
TAGAGTT412550.025.91243425
ATAGAGT410050.025.8346124
ACTCATT687000.025.29428135
Produced by FastQC (version 0.11.5)