FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005744244

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744244
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48484261
Sequences flagged as poor quality0
Sequence length50-51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10817072.231047720826352No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1875730.3868740002039012No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG1612360.33255327950651864No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA840760.17340885117337357No Hit
CACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA573050.1181929946297418No Hit
CTTTTATACATGCATTCCTTGGAAAGCTGAACAAAATGAGTGAAAACTCTA505630.1042874511380095No Hit
CCTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTA493030.10168866964889904No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGCG5700.031.18365711
TAGCGCG2600.031.15508834
TCGTACG6000.031.1245066
CGTACGC6850.028.5761897
TACGCTC7200.027.18858534
CGTCGAA15200.026.1987325
AACTCTA315800.024.59911245
CGAACGT7100.023.44897820
CTAGTTG26850.023.21321729
CGATCGT7850.022.93085138
CGCTAGG9450.022.855461
TTACGCG6700.022.16254818
CGATACG13100.021.8114194
ACGATAC15350.021.5456453
CGTCGTT29750.021.48326142
CCGATTA8000.021.0922414
TCACGTA15500.020.90405830
CGCGAAT9850.020.55966437
CGCGGAT15400.020.45304122
TACGCGC8750.020.31329512
Produced by FastQC (version 0.11.5)