FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005744248

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744248
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52550408
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTCTA178550.016.75257345
CATGCAT134000.012.9042559
CGTAACG13550.012.43899442
ATACATG144050.012.0039596
TATATAC97300.011.7777227
GTTGTCG35600.011.48366526
ATAGGGG116400.011.25135638
AAACTCT281850.011.23456644
TGCATTC159550.010.91098711
ATATATA114700.010.8021946
TACATGC162800.010.5656837
CCATATA108550.010.5361874
GGCATAG120550.010.45454635
TTCCATA137600.010.3497522
AGCTTAG128250.09.90043745
TCCATAT145450.09.7139553
AATGAGT344600.09.648416535
ATATACA128550.09.6207118
TGAGTGA361850.09.61063137
CATATAT123800.09.5865825
Produced by FastQC (version 0.11.5)