FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005744249

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744249
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65305436
Sequences flagged as poor quality0
Sequence length34-51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT882410.1351204515348462No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG658600.10084918505099637No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG57850.025.3983336
TCGACGA69200.020.80917
GTTCGAC69900.020.6660065
CGACGAG68350.020.5411388
CGGTATA101750.018.75103620
GAACGAT272850.017.5314645
ACCGCGT263050.016.8509086
GACCGCG264050.016.727575
AGACCGC269100.016.7063224
TCACGCT232750.016.5304558
AGTTCGA90800.016.2809054
CCGGCTT276300.016.1985652
AACGCTA234900.016.1487323
ACGCTAC236850.016.04427324
CCGCGTT276000.016.0433647
CAGACCG282950.016.000113
ACGCGTC29750.015.80705740
CGTTACG31650.015.71022321
CGCGTTC284500.015.5798188
CACGCGT32900.015.52514439
Produced by FastQC (version 0.11.5)