FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005744251

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744251
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78742464
Sequences flagged as poor quality0
Sequence length25-101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA880400.010.94341994-95
ACGTCTG1060600.010.29313494-95
ACACGTC1279700.09.78674694-95
CACGTCT1228100.09.63841894-95
CACACGT1502600.09.38351894-95
GCACACG1754800.09.054028594-95
TCGGAAG3780300.08.48250494-95
ATCGGAA4019650.08.4301894-95
AGAGCAC2606700.08.40360494-95
CGGAAGA3529450.08.39459294-95
GATCGGA4412050.08.33923894-95
TCTGAAC996000.08.27323794-95
AGCACAC2155950.08.19086594-95
GTCTGAA1095300.08.18205494-95
AGATCGG4892300.08.17295394-95
GAGCACA2447950.07.99840194-95
TAGATCG1362500.07.84358694-95
AAGAGCA3125850.07.69039694-95
GAAGAGC3464950.07.51363594-95
CAGATCG1694600.07.50596594-95
Produced by FastQC (version 0.11.5)