FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005744253

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744253
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52443238
Sequences flagged as poor quality0
Sequence length34-51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1249330.23822518357848158No Hit
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAG801900.15290817855297187No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG665720.126941055775389No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG41000.020.2502356
GACCGCG318700.019.789145
CGGTATA53900.019.57738320
AGACCGC327550.019.5423184
ACCGCGT324400.019.5177976
CAGACCG333050.019.3141733
CGACGAG42100.018.9194778
TCGGCGC113700.018.03369145
TCGACGA44750.017.8996497
TCAGACC376200.017.8882982
CCGCGTT356950.017.7253767
CGCGTTC368850.017.159638
AACGACG25550.016.0269116
CTCAGAC460350.015.9380081
GTCCGTT43500.015.0513391
GTTCGAC55700.015.0270465
CGTACGC140900.014.9947945
GCGTTCT427500.014.8370289
CCGTACG143900.014.6816954
CGTTACG21600.014.37460521
Produced by FastQC (version 0.11.5)