FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005744254

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744254
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52443238
Sequences flagged as poor quality0
Sequence length26-51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG780850.1488943150306623No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG731390.13946316587087929No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA720300.13734849858050335No Hit
CCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGG560570.10689080639910145No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG285050.013.94362145
GGAGGTC379550.013.7122074
GTCACTC376100.013.496238
GGGAGGT417050.013.3155973
GGTCACT383450.013.2964517
GGCATAG308900.012.89246135
GAGGTCA417450.012.7959475
ATAGGGG316200.012.73699838
ACTCTCC405450.012.6850711
AGGTCAC417700.012.6156586
GAGCTTA333550.011.92865944
ACCCGGC72100.011.890115
TGGCATA339150.011.80911434
TCACTCT445000.011.5427529
CACTCTC468150.010.82754810
CATAGGG384750.010.37998837
TCTCCCC501450.010.36426913
TCAGACG109400.010.2638152
GCTCTGT520900.010.17597722
GCATAGG394700.010.17523636
Produced by FastQC (version 0.11.5)