FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005744256

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744256
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55881581
Sequences flagged as poor quality0
Sequence length25-51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG886650.15866587597083195No Hit
CGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTG649690.11626192179494707No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATTC597210.10687063417192867No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACT575270.10294447467404332No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGGT432150.014.5434663
GGAGGTC434800.014.1908574
GTCACTC426200.013.887738
GGTCACT431300.013.7914317
TCAGACG146900.013.3272172
AGCTTAG348050.013.17331845
ACTCTCC453100.013.07789311
GAGGTCA478750.013.0174965
AGGTCAC475200.012.9339636
CCGTACG32850.012.6041384
GGCATAG362250.012.58404335
ATAGGGG374150.012.48477838
CGTACGC34000.012.4404315
TGCCCGT260500.012.13098944
TGGGAGG402250.012.1284472
CGTGGCG310000.011.9048561
TCACTCT505650.011.8302029
TGGCATA390150.011.7358734
GAGCTTA396400.011.55493744
CACTCTC519250.011.32958410
Produced by FastQC (version 0.11.5)